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KMID : 0545120110210121287
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 12 p.1287 ~ p.1293
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Biochemical Characterization of the Exopolysaccharide Purified from Laetiporus sulphureus Mycelia
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Seo Min-Jeong
Kang Byoung-Won
Park Jeong-Uck
Kim Min-Jeong
Lee Hye-Hyeon
Choi Yung-Hyun
Jeong Yong-Kee
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Abstract
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The extracellular polysaccharide (EPS) was isolated from mycelial cultures of Laetiporus sulphureus var. miniatus and purified by DEAE cellulose and Sephadex G-50 column chromatography. The purified EPS (EPS-2-1) was composed of only glucose units and its molecular mass was 6.95 kDa. The chemical structure of EPS-2-1 consisted of a main chain containing (1→4)-Glcp units with branches at the C-6 position of the chain carrying -Glcp-(1→4)-linked residues. The effect of purified EPS on immunomodulatory genes and proteins of the Bcl-2 family was observed using cultured U937 human leukemia cells. Of note, the levels of Bax and Bad proteins treated with the EPS (4 mg/ml) were approximately 23- and 18-times higher than those in non-treated cells, respectively. These results may suggest that the EPS purified from the mushroom L. sulphureus is associated with the activation of immunomodulatory mediators, Bax and Bad proteins.
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KEYWORD
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Extracellular polysaccharide, Laetiporus sulphureus, immunomodulatory proteins, mycelial culture
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